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Galantos HEParanase Assay:

 



Assay accuracy

CV values (intra-assay) are in the range of 2-3 % for manually performed assays and < 2 % for automated assays.

Assay Features :

heparanase diagramm02

Fig. 2: Dose-dependent degradation of labeled heparan sulfate (substrate) by heparanase. (10 µl of purified heparanase are equivalent to 0.1 µU or 0.15 ng of recombinant heparanase).

 

heparanase diagramm01

Fig. 3: Dose-dependent inhibition of heparanase enzyme activity by heparin, a known inhibitor of heparanase. The calculated IC50 was 4.4 ng / ml.

Recommended Concentration Range for Inhibitors:

Potent inhibitors: 0.5 - 10 ng / ml
Less potent inhibitors: 10 - 100 ng / ml

(Based on 100 µl / well for 96-well microplates and 20 µl / well for 384 well plates (e.g. for 384 well plates 0.01 - 0.2 ng / well of sample are needed))